WDR61 Rabbit Polyclonal Antibody

CAT#: TA383312

WDR61 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using WDR61 antibody (TA383312) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 40s.

• 

  Immunohistochemistry of paraffin-embedded Human placenta using WDR61 Rabbit pAb (TA383312) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• 

  Immunohistochemistry of paraffin-embedded Rat brain using WDR61 Rabbit pAb (TA383312) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB,1:200 - 1:2000
  IHC,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human WDR61 (NP_079510.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 33kDa
• Gene Name: WD repeat domain 61
• Database Link:
  Entrez Gene 80349 Human
  UniProt ID Q9GZS3
• Background: WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]).[supplied by OMIM, Mar 2008]
• Synonyms: REC14; SKI8