Apg3 (ATG3) Rabbit Monoclonal Antibody [Clone ID: R06-9E7]

CAT#: TA383765

ATG3 Rabbit monoclonal Antibody

Product Images

Western blot detection of ATG3 in K562,Rat Brain,C6,3T3,Hela cell lysates using ATG3 Rabbit mAb(1:1000 diluted).Predicted band size:36kDa.Observed band size:40kDa.

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  Immunocytochemistry of ATG3(green) in Hela cells using ATG3 Rabbit mAb at dilution 1/50, and DAPI(blue)

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  Immunohistochemistry of ATG3 in paraffin-embedded Human tonsil using ATG3 Rabbit mAb at dilution 1/5

Specifications

Product Data

• Clone Name: R06-9E7
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/2000-1/10000
  IHC: 1/50
  ICC/IF: 1/500
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human ATG3
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 36 kDa; Observed MW: 40 kDa
• Gene Name: autophagy related 3
• Database Link:
  Entrez Gene 64422 Human
  UniProt ID Q9NT62
• Background: Swiss-Prot Acc.Q9NT62.E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
• Synonyms: 2610016C12Rik; APG3; APG3-LIKE; APG3L; Apg3p; DKFZp564M1178; FLJ22125; hApg3; MGC15201; PC3-96