ATM Mouse Monoclonal Antibody [Clone ID: 9F7-2B5-5G2]

CAT#: TA383777

ATM (9F7) Mouse monoclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using ATM Mouse mAb diluted at 1:200

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  Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATM Mouse mAb diluted at 1:200.

Specifications

Product Data

• Clone Name: 9F7-2B5-5G2
• Applications: IHC
• Recommended Dilution: IHC: 1/100-200
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide conjugated to KLH.
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 350kDa
• Gene Name: ATM serine/threonine kinase
• Database Link:
  Entrez Gene 472 Human
  UniProt ID Q13315
• Background: Swiss-Prot Acc.Q13315.Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.
• Synonyms: AT1; ATA; ATC; ATD; ATDC; ATE; DKFZp781A0353; MGC74674; TEL1; TELO1