SERCA2 (ATP2A2) Rabbit Monoclonal Antibody [Clone ID: R09-1G2]

CAT#: TA383783

SERCA2 ATPase Rabbit monoclonal Antibody

Product Images

Western blot detection of SERCA2 ATPase in K562,Hela cell lysates using SERCA2 ATPase Rabbit mAb(1:1000 diluted).Predicted band size:115kDa.Observed band size:115kDa.

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  Immunocytochemistry of SERCA2 ATPase(green) in Hela cells using SERCA2 ATPase Rabbit mAb at dilution 1/50, and DAPI(blue)

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  Immunohistochemistry of SERCA2 ATPase in paraffin-embedded Human colon cancer tissue using SERCA2 ATPase Rabbit mAb at dilution 1/5

Specifications

Product Data

• Clone Name: R09-1G2
• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/2000-1/10000
  IHC: 1/20
  ICC/IF: 1/50-1/100
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human SERCA2 ATPase
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 115 kDa; Observed MW: 115 kDa
• Gene Name: ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
• Database Link:
  Entrez Gene 488 Human
  UniProt ID P16615
• Background: Swiss-Prot Acc.P16615.This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle (PubMed:16402920). Acts as a regulator of TNFSF11-mediated Ca2+ signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation .
• Synonyms: ATP2B; DAR; DD; DKFZp686P0211; FLJ20293; FLJ38063; MGC45367; SERCA2