p21 (CDKN1A) Rabbit Polyclonal Antibody

CAT#: TA383974

p21 Rabbit polyclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,p21 Polyclonal Antibody was diluted at 1:2(4°C,overnight). 2, Sodium citrate pH 6. was used for antibody retrieval(>98°C,2min). 3,Secondary antibody was diluted at 1:2(room tempeRature, 3min). Negative control was used by secondary antibody only.

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  Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,p21 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

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  Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,p21 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

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  Immunofluorescence analysis of mouse-kidney tissue. 1,p21 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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  Immunofluorescence analysis of rat-lung tissue. 1,p21 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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  Western Blot analysis of various cells using p21 Polyclonal Antibody

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Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: IF: 1/50-200
  WB: 1/500-1/2000
  ELISA: 1/20000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from human p21 Cip1. AA range:111-160
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):26
• Gene Name: cyclin-dependent kinase inhibitor 1A
• Database Link:
  Entrez Gene 1026 Human
  UniProt ID P38936
• Background: Swiss-Prot Acc.P38936.
• Synonyms: CAP20; CDKN1; CIP1; MDA-6; MDA6; OTTHUMP00000016298; P21; p21CIP1; p21Cip1/Waf1; PIC1; SDI1; WAF1