p27 KIP 1 (CDKN1B) Rabbit Monoclonal Antibody [Clone ID: R09-6C3]

CAT#: TA383979

p27 Kip 1 Rabbit monoclonal Antibody

Product Images

Western blot detection of p27 KIP 1 in 3T3,Hela cell lysates using p27 KIP 1 Rabbit mAb(1:1000 diluted).Predicted band size:22kDa.Observed band size:27kDa.

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  Immunohistochemistry of p27 KIP 1 in paraffin-embedded Human breast cancer tissue using p27 KIP 1 Rabbit mAb at dilution 1/20

Specifications

Product Data

• Clone Name: R09-6C3
• Applications: IHC, IP, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/20
  IP: 1/20
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human p27 KIP 1
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 22 kDa; Observed MW: 27 kDa
• Gene Name: cyclin-dependent kinase inhibitor 1B
• Database Link:
  Entrez Gene 1027 Human
  UniProt ID P46527
• Background: Swiss-Prot Acc.P46527.Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
• Synonyms: CDKN4; KIP1; MEN1B; MEN4; P27KIP1