DCLRE1C Rabbit Polyclonal Antibody

CAT#: TA384086

Phospho-Artemis (Ser516) Rabbit polyclonal Antibody

Product Images

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Artemis (Phospho-Ser516) Antibody. The picture on the right is blocked with the phospho peptide.

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  Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 30', using Artemis (Phospho-Ser516) Antibody. The lane on the right is blocked with the phospho peptide.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/100-1/300
  ELISA: 1/5000
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized peptide derived from human Artemis around the phosphorylation site of Ser516. AA range:482-531 (Phosphorylated)
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW (kDa):78
• Database Link:
  UniProt ID Q96SD1
• Background: Swiss-Prot Acc.Q96SD1.Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.