DDX21 Rabbit Monoclonal Antibody [Clone ID: R04-4J2]

CAT#: TA384091

DDX21 Rabbit monoclonal Antibody

Product Images

Western blot detection of DDX21 in Hela cell lysates using DDX21 Rabbit mAb(1:1000 diluted).Predicted band size:87kDa.Observed band size:87kDa.

Specifications

Product Data

• Clone Name: R04-4J2
• Applications: IF, WB
• Recommended Dilution: WB: 1/1000
  ICC/IF: 1/100
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human DDX21
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 87 kDa; Observed MW: 87 kDa
• Gene Name: DEAD-box helicase 21
• Database Link:
  Entrez Gene 9188 Human
  UniProt ID Q9NR30
• Background: Swiss-Prot Acc.Q9NR30.RNA helicase that acts as a sensor of the transcriptional status of both RNA polymerase (Pol) I and II: promotes ribosomal RNA (rRNA) processing and transcription from polymerase II (Pol II) (PubMed:25470060). Binds various RNAs, such as rRNAs, snoRNAs, 7SK and, at lower extent, mRNAs (PubMed:25470060). In the nucleolus, localizes to rDNA locus, where it directly binds rRNAs and snoRNAs, and promotes rRNA transcription, processing and modification. Required for rRNA 2'-O-methylation, possibly by promoting the recruitment of late-acting snoRNAs SNORD56 and SNORD58 with pre-ribosomal complexes (PubMed:25470060, PubMed:25477391). In the nucleoplasm, binds 7SK RNA and is recruited to the promoters of Pol II-transcribed genes: acts by facilitating the release of P-TEFb from inhibitory 7SK snRNP in a manner that is dependent on its helicase activity, thereby promoting transcription of its target genes (PubMed:25470060). Functions as cofactor for JUN-activated transcription: required for phosphorylation of JUN at 'Ser-77' (PubMed:11823437, PubMed:25260534). Can unwind double-stranded RNA (helicase) and can fold or introduce a secondary structure to a single-stranded RNA (foldase) (PubMed:9461305). Involved in rRNA processing (PubMed:14559904, PubMed:18180292). May bind to specific miRNA hairpins (PubMed:28431233).
• Synonyms: DKFZp686F21172; Gu-alpha; GUA; GURDB; RH-II/GU; RH-II/GuA