H3.3A (H3F3A) Rabbit Polyclonal Antibody

CAT#: TA384375

Histone H3.3 Rabbit polyclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded mouse stomach, using Histone H3.3 Antibody .

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  Western blot analysis of Histone H3.3 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1/500-1/1000
  IHC: 1/500-1/1000
  ICC/IF: 1/500-1/1000
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human Histone H3.3
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 15kDa
• Gene Name: H3 histone, family 3A
• Database Link:
  Entrez Gene 3020 Human
  UniProt ID P84243
• Background: Swiss-Prot Acc.P84243.Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template.
• Synonyms: H3.3A; H3.3B; H3F3; MGC87782; MGC87783; OTTHUMP00000035619; OTTHUMP00000035621