H3.3A (H3F3A) Rabbit Monoclonal Antibody [Clone ID: R04-5J3]

CAT#: TA384376

Histone H3.3 Rabbit monoclonal Antibody

Product Images

Western blot detection of Histone H3.3 in K562,C6,3T3 cell lysates using Histone H3.3 Rabbit mAb(1:1000 diluted).Predicted band size:15kDa.Observed band size:15kDa.

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  Immunohistochemistry of Histone H3.3 in paraffin-embedded Human lung cancer tissue using Histone H3.3 Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R04-5J3
• Applications: IHC, WB
• Recommended Dilution: WB: 1/1000
  IHC: 1/200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Histone H3.3
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 15 kDa; Observed MW: 15 kDa
• Gene Name: H3 histone, family 3A
• Database Link:
  Entrez Gene 3020 Human
  UniProt ID P84243
• Background: Swiss-Prot Acc.P84243.Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
• Synonyms: H3.3A; H3.3B; H3F3; MGC87782; MGC87783; OTTHUMP00000035619; OTTHUMP00000035621