HSF1 Rabbit Monoclonal Antibody [Clone ID: R08-4I1]

CAT#: TA384455

Phospho-HSF1 (Ser326) Rabbit monoclonal Antibody

Product Images

Western blot detection of Phospho-HSF1 (Ser326) in K562,Hela cell lysates using Phospho-HSF1 (Ser326) Rabbit mAb(1:1000 diluted).Predicted band size:57kDa.Observed band size:82kDa.

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  Immunocytochemistry of HSF1 (Phospho-Ser326)(green) in Hela cells using HSF1 (Phospho-Ser326) Rabbit mAb at dilution 1/50, and DAPI(blue)

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  Immunohistochemistry of HSF1 (Phospho-Ser326) in paraffin-embedded Human breast cancer tissue using HSF1 (Phospho-Ser326) Rabbit mAb at dilution 1/2

Specifications

Product Data

• Clone Name: R08-4I1
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/1000-1/5000
  IHC: 1/20
  ICC/IF: 1/500
  IP: 1/20
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Ser326 of human HSF1 (Phosphorylated)
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 57 kDa; Observed MW: 82 kDa
• Gene Name: heat shock transcription factor 1
• Database Link:
  Entrez Gene 3297 Human
  UniProt ID Q00613
• Background: Swiss-Prot Acc.Q00613.Function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones heat shock proteins (HSPs) that protect cells from cellular insults' damage (PubMed:1871105, PubMed:11447121, PubMed:1986252, PubMed:7760831, PubMed:7623826, PubMed:8946918, PubMed:8940068, PubMed:9341107, PubMed:9121459, PubMed:9727490, PubMed:9499401, PubMed:9535852, PubMed:12659875, PubMed:12917326, PubMed:15016915, PubMed:25963659, PubMed:26754925). In unstressed cells, is present in a HSP90-containing multichaperone complex that maintains it in a non-DNA-binding inactivated monomeric form (PubMed:9727490, PubMed:11583998, PubMed:16278218). Upon exposure to heat and other stress stimuli, undergoes homotrimerization and activates HSP gene transcription through binding to site-specific heat shock elements (HSEs) present in the promoter regions of HSP genes (PubMed:1871105, PubMed:1986252, PubMed:8455624, PubMed:7935471, PubMed:7623826, PubMed:8940068, PubMed:9727490, PubMed:9499401, PubMed:10359787, PubMed:11583998, PubMed:12659875, PubMed:16278218, PubMed:25963659, PubMed:26754925). Activation is reversible, and during the attenuation and recovery phase period of the HSR, returns to its unactivated form (PubMed:11583998, PubMed:16278218). Binds to inverted 5'-NGAAN-3' pentamer DNA sequences (PubMed:1986252, PubMed:26727489). Binds to chromatin at heat shock gene promoters (PubMed:25963659). Plays also several other functions independently of its transcriptional activity. Involved in the repression of Ras-induced transcriptional activation of the c-fos gene in heat-stressed cells (PubMed:9341107). Positively regulates pre-mRNA 3'-end processing and polyadenylation of HSP70 mRNA upon heat-stressed cells in a symplekin (SYMPK)-dependent manner (PubMed:14707147). Plays a role in nuclear export of stress-induced HSP70 mRNA (PubMed:17897941). Plays a role in the regulation of mitotic progression (PubMed:18794143). Plays also a role as a negative regulator of non-homologous end joining (NHEJ) repair activity in a DNA damage-dependent manner (PubMed:26359349). Involved in stress-induced cancer cell proliferation in a IER5-dependent manner (PubMed:26754925).
• Synonyms: HSTF1