Cpn10 (HSPE1) Rabbit Monoclonal Antibody [Clone ID: R02-3K6]

CAT#: TA384471

Cpn10 Rabbit monoclonal Antibody

Product Images

Western blot detection of Cpn10 in K562,Hela cell lysates using Cpn10 Rabbit mAb(1:1000 diluted).Predicted band size:11kDa.Observed band size:11kDa.

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  Immunohistochemistry of Cpn10 in paraffin-embedded Human colon cancer tissue using Cpn10 Rabbit mAb at dilution 1/50

Specifications

Product Data

• Clone Name: R02-3K6
• Applications: IHC, IP, WB
• Recommended Dilution: WB: 1/2000-1/10000
  IHC: 1/50-1/200
  IP: 1/20-1/50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human Cpn10
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 11 kDa; Observed MW: 11 kDa
• Gene Name: heat shock protein family E (Hsp10) member 1
• Database Link:
  Entrez Gene 3336 Human
  UniProt ID P61604
• Background: Swiss-Prot Acc.P61604.Co-chaperonin implicated in mitochondrial protein import and macromolecular assembly. Together with Hsp60, facilitates the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix (PubMed:7912672, PubMed:1346131, PubMed:11422376). The functional units of these chaperonins consist of heptameric rings of the large subunit Hsp60, which function as a back-to-back double ring. In a cyclic reaction, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by the binding of ATP and association with 2 heptameric rings of the co-chaperonin Hsp10. This leads to sequestration of the substrate protein in the inner cavity of Hsp60 where, for a certain period of time, it can fold undisturbed by other cell components. Synchronous hydrolysis of ATP in all Hsp60 subunits results in the dissociation of the chaperonin rings and the release of ADP and the folded substrate protein (Probable).
• Synonyms: CPN10; EPF; GROES; Hsp10