LPP Mouse Monoclonal Antibody [Clone ID: 6F6-C1-B6]

CAT#: TA384675

LPP (6F6) Mouse monoclonal Antibody

Product Images

Immunohistochemical analysis of paraffin-embedded human vessels tissues using LPP mouse mAb.

• 

  Immunohistochemical analysis of paraffin-embedded human small intestine using LPP mouse mAb with DAB staining.

• 

  Immunoprecipitation analysis of Hela cell lysates using LPP (6F6) Mouse mAb.

• 

  Western blot detection of LPP in A549,MCF7,C6 and Hela cell lysates using LPP mouse mAb (1:5000 diluted).Predicted band size:66KDa.Observed band size:75KDa.

• 

  Confocal immunofluorescence analysis of COS cells using LPP mouse mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

• 

  Confocal immunofluorescence analysis of Hela cells using LPP mouse mAb (green). Red: Actin filaments have been labeled using DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

Show all 6 images

Specifications

Product Data

• Clone Name: 6F6-C1-B6
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/200-1/1000
  ICC: 1/200-1/1000
  IP: 1/20
• Reactivities: Hamster, Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human LPP expressed in E. Coli.
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Ascitic Fluid
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 66kDa
• Gene Name: LIM domain containing preferred translocation partner in lipoma
• Database Link:
  Entrez Gene 4026 Human
  UniProt ID Q93052
• Background: Swiss-Prot Acc.Q93052.LIM domain containing preferred translocation partner in lipoma. The Zyxin family of proteins contains five members, Ajuba, LIMD1, LPP,TRIP6 and Zyxin. LPP (LIM-containing lipoma-preferred partner), a LIM domain-containing scaffolding protein contains three LIM domains at its carboxyterminus, which are preceded by a proline-rich pre-LIM region containing a number of protein interaction domains. LPP, an 80 kDa protein, localizes to sites of cell adhesion, such as focal adhesions and cell-cell contacts,and shuttles to the nucleus where it has transcriptional activation capacity.The human LPP gene maps to chromosomal location 3q28, and preferentiallytranslocates to the HMGIC gene in a subclass of human benign mesenchymal tumors known as lipomas.
• Synonyms: LPP