RAF1 Rabbit Polyclonal Antibody

CAT#: TA385123

Phospho-Raf1 (Ser259) Rabbit polyclonal Antibody

Product Images

Western blot analysis of Phospho-Raf1 (S259) expression in (1) 293T cell lysate; (2) 293T cell lysates treated with Okadaic Acid + Calyculin A.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1/500-1/1000
  IHC: 1/50-1/100
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human Phospho-Raf1 (S259) (Phosphorylated)
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 73kDa
• Gene Name: Raf-1 proto-oncogene, serine/threonine kinase
• Database Link:
  Entrez Gene 5894 Human
  UniProt ID P04049
• Background: Swiss-Prot Acc.P04049.Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
• Synonyms: C-RAF; CRAF; NS5; RAF; Raf-1