RHOC Rabbit Polyclonal Antibody

CAT#: TA385167

RhoA/B/C Rabbit polyclonal Antibody

Product Images

Western blot analysis of Rho A + B + C expression in SHSY-5Y and Rat Brain cell lysates using Rho A + B + C antibody at 1/1000 dilution.Predicted band size:22KDa.Observed band size:22KDa.

• 

  Western blot analysis of Rho A + B + C expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate.

• 

  Immunofluorescent analysis of Hela cells, using Rho A + B + C Antibody .

• 

  Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rho A + B + C Antibody .

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1/500-1/2000
  IHC: 1/500-1/1000
  ICC/IF: 1/100-1/500
  FC: 1/200
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthesized peptide derived from human Rho A + B + C
• Formulation: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: lot specific
• Purification: Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: 22kDa
• Gene Name: ras homolog family member C
• Database Link:
  Entrez Gene 389 Human
  UniProt ID P61586
• Background: Swiss-Prot Acc.P61586,P62745,P08134.RhoA,RhoB,RhoC is a small G protein of the Rho family. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. The three mammalian Rho proteins (A, B and C) are approximately 30% homologous to Ras and are expressed in a wide range of cell types. Both Ras p21 and Rho p21, as well as other members of the Ras superfamily, contain a carboxy terminal CAAX sequence (C, cysteine; A, aliphatic amino acid; X, any amino acid) which in the case of Ras has been shown to be essential for correct localization and function.
• Synonyms: ARH9; ARHC; H9; MGC1448; MGC61427; OTTHUMP00000013802; OTTHUMP00000013805; OTTHUMP00000013807; RHOH9