SET Rabbit Monoclonal Antibody [Clone ID: R04-5E8]

CAT#: TA385230

SET Rabbit monoclonal Antibody

Product Images

Western blot detection of SET in K562,Hela cell lysates using SET Rabbit mAb(1:1000 diluted).Predicted band size:34kDa.Observed band size:39kDa.

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  Immunocytochemistry of SET(green) in Hela cells using SET Rabbit mAb at dilution 1/50, and DAPI(blue)

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  Immunohistochemistry of SET in paraffin-embedded Human lung cancer tissue using SET Rabbit mAb at dilution 1/5

Specifications

Product Data

• Clone Name: R04-5E8
• Applications: IF, IHC, IP, WB
• Recommended Dilution: WB: 1/2000-1/10000
  IHC: 1/50
  ICC/IF: 1/200
  IP: 1/20
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human SET
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 34 kDa; Observed MW: 39 kDa
• Gene Name: SET nuclear proto-oncogene
• Database Link:
  Entrez Gene 6418 Human
  UniProt ID Q01105
• Background: Swiss-Prot Acc.Q01105.Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.
• Synonyms: 2PP2A; I-2PP2A; I2PP2A; IGAAD; IPP2A2; PHAPII; TAF-I; TAF-IBETA