SF3B3 Rabbit Monoclonal Antibody [Clone ID: R07-2K8]

CAT#: TA385232

SF3B3 Rabbit monoclonal Antibody

Product Images

Western blot detection of SF3B3 in SHSY-5Y,C6 cell lysates using SF3B3 Rabbit mAb(1:1000 diluted).Predicted band size:136KDa.Observed band size:136KDa.

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  Immunocytochemistry of SF3B3(green) in Hela cells using SF3B3 Rabbit mAb at dilution 1/50, and DAPI(blue)

Specifications

Product Data

• Clone Name: R07-2K8
• Applications: IF, WB
• Recommended Dilution: WB: 1/1000
  ICC/IF: 1/50
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human SF3B3
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 136 kDa; Observed MW: 136 kDa
• Gene Name: splicing factor 3b subunit 3
• Database Link:
  Entrez Gene 23450 Human
  UniProt ID Q15393
• Background: Swiss-Prot Acc.Q15393.Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome (PubMed:10490618, PubMed:10882114, PubMed:27720643, PubMed:28781166). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).
• Synonyms: KIAA0017; RSE1; SAP130; SF3b130; STAF130