SMAD2 Mouse Polyclonal Antibody

CAT#: TA385275

Smad2/3 (6F7) Mouse monoclonal Antibody

Product Images

Western blot analysis of extracts from C6,MCF7,Jurkat and 3T3 cell lysates using Smad2/3 mouse mAb (1:500 diluted).Predicted band size:52/6KDa.Observed band size:52/6KDa.

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  Immunohistochemical analysis of paraffin-embedded Human Live rTissue using Smad2/3 mouse mAb diluted at 1:200.

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  Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma using Smad2/3 Mouse mAb diluted at 1:200.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1/1000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant Protein of Smad3
• Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.03% Proclin 300, pH 7.3.
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Observed MW(kDa):52/60
• Gene Name: SMAD family member 2
• Database Link:
  Entrez Gene 4087 Human
  UniProt ID P84022
• Background: Swiss-Prot Acc.P84022.Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 . Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses .
• Synonyms: hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2; MGC22139; MGC34440