ULK3 Rabbit Monoclonal Antibody [Clone ID: R08-7J3]

CAT#: TA385524

ULK3 Rabbit monoclonal Antibody

Product Images

Western blot detection of ULK3 in K562,Hela cell lysates using ULK3 Rabbit mAb(1:1000 diluted).Predicted band size:53kDa.Observed band size:53kDa.

Specifications

Product Data

• Clone Name: R08-7J3
• Applications: WB
• Recommended Dilution: WB: 1/1000-1/5000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: A synthetic peptide of human ULK3
• Formulation: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
• Concentration: lot specific
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
• Stability: 1 year
• Predicted Protein Size: Calculated MW: 53 kDa; Observed MW: 53 kDa
• Gene Name: unc-51 like kinase 3
• Database Link:
  Entrez Gene 25989 Human
  UniProt ID Q6PHR2
• Background: Swiss-Prot Acc.Q6PHR2.Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3). Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
• Synonyms: DKFZP434C131; FLJ90566