IL2 Receptor alpha (IL2RA) Rabbit Monoclonal Antibody [Clone ID: Basiliximab]

CAT#: TA385814

Recombinant Anti-IL-2R alpha (CD25) (Clone Basiliximab)


USD 523.00

2 Weeks*

Size
    • 200 ug

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Specifications

Product Data
Clone Name Basiliximab
Applications FC, IF, IHC, WB
Reactivities Human, Monkey
Modifications This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Host Rabbit
Isotype IgG, kappa
Clonality Monoclonal
Immunogen Basiliximab was prepared by immunizing BALB/c mice with CTC 16 cells.
Specificity Basiliximab recognises the human IL-2R and binds to the epitope (116)ERIYHFV(122) within the extracellular domain of the CD25 subunit – binding is abolished when 2 or more residues within the epitope are mutated. This epitope overlaps with the interaction site of CD25 and IL-2. CD25 is found in the high-affinity IL-2R (αβγc) – T cells only express CD25 upon activation. IL-2 binding to IL-2R on T cells modulates proliferation and differentiation.

Basiliximab is derived from the murine anti-Tac antibody. For the clinically used format, the variable regions from this antibody were grafted onto the sequence of the human IgG1κ constant regions. Basiliximab binding to CD25 competitively blocks the interaction of IL-2R with its ligand IL-2 on the surface of T cells. Blocking this interaction prevents T cell replication and T cell-mediated B cell activation. Basiliximab binding suppresses the immune system inhibits the pathway activating the cellular immune response to allograft rejection. IL-2 signalling suppression can additionally prevent acute graft-versus-host disease, autoimmune disorders and malignancies such as T cell leukemias (Binder et al, 2007). Basiliximab has also been used in FC, IF, IHC and WB studies, which enables the detection of CD25.
Formulation PBS with 0.02% Proclin 300.
Concentration lot specific
Conjugation Unconjugated
Storage Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
Stability 3 years from dispatch.
Gene Name interleukin 2 receptor subunit alpha
Synonyms CD25; IDDM10; IL-2-RA; IL2-RA; IL2R; p55; TCGFR
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.