Cardiac Troponin I (TNNI3) Mouse Monoclonal Antibody [Clone ID: scFv 180]

CAT#: TA385951

Recombinant Anti-Cardiac Troponin I (Clone scFv 180)

Product Images

Western Blot using anti-Cardiac Troponin I antibody scFv 180 (Ab00434) Human heart lysate (35µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of scFv 180 (TA385951) at 0.001 µg/ml before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for Cardiac Troponin I is 24.0 kDa. TA385951 successfully detected Cardiac Troponin I in human heart lysate.

Specifications

Product Data

• Clone Name: scFv 180
• Applications: WB
• Reactivities: Human
• Modifications: This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Chicken scFv format, for improved compatibility with existing reagents, assays and techniques.
• Host: Mouse
• Isotype: IgG1, lambda
• Clonality: Monoclonal
• Immunogen: Cardiac Troponin I peptide (KISASRKLQLKT).
• Specificity: This antibody binds specificially to the cardiac Troponin I Peptide KISASRKLQLKT. This epitope is 100% conserved among a great variety of mammalian species, including most monkeys, Squirrel, Hamster, Mouse, Rat, Rabbit, Whales, Mole-Rat, Antelope and several other mammals.
  
  Troponin I is the inhibitory subunit of troponin, the thin filament complex that confers calcium-sensitivity to muscle-actomyoisin ATPase activity. Cardiac Troponin I in the blood is also a marker for several heart muscle damages including myocardial infarction.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: troponin I3, cardiac type
• Database Link:
  Entrez Gene 7137 Human
  UniProt ID P19429
• Synonyms: CMD2A; CMH7; cTnI; MGC116817; RCM1; TNNC1