CD40L (CD40LG) Rabbit Monoclonal Antibody [Clone ID: hu5c8 (Ruplizumab)]

CAT#: TA385963

Recombinant Anti-CD40L (Clone hu5c8 (Ruplizumab))

Product Images

Flow-cytometry using the anti-CD40L research biosimilar antibody Ruplizumab (hu5c8, Ab00447) Human lymphocytes were stained with an isotype control (Ab00102-23.0, panel A) or the rabbit-chimeric version of Ruplizimab (TA385963, panel B) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: hu5c8 (Ruplizumab)
• Applications: FC, WB
• Reactivities: Human
• Modifications: NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: Human CD40L
• Specificity: The antibody binds to CD40L with an IC50 of 0.845 ug/ml.
  
  The antibody binds specifically to CD40L, a surface receptor expressed on activated T cells which acts as a costimulatory molecule to trigger immune responses. The antibody neutralises CD40L function, as it blocks the interaction between CD40 and CD40L. When injected into cynomolgus monkeys, the original therapeutic antibody was found to have a T1/2 of 531 ± 155 hours.
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: CD40 ligand
• Database Link:
  Entrez Gene 959 Human
  UniProt ID P29965
• Synonyms: CD40-L; CD40L; CD154; gp39; hCD40L; HIGM1; IGM; IMD3; T-BAM; TNFSF5; TRAP