Fcer1a Rabbit Monoclonal Antibody [Clone ID: Mar-1]

CAT#: TA386261

Recombinant Anti-FceR1 alpha (Clone Mar-1)

Product Images

Flow-cytometry using the anti-FceR1 alpha antibody Mar-1 (Ab01187) Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG-chimeric version of Mar-1 (TA386261, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: Mar-1
• Applications: FC, IHC, IP
• Reactivities: Mouse
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Armenian Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Clonality: Monoclonal
• Specificity: This antibody specifically targets the non-signalling alpha chain of the FceR1 complex.
  
  This antibody has been used in flow cytometric analysis of FceR1 alpha cell surface expression by basophils (Min et al, 2004; Chen et al, 2009), and immunohistochemical analysis of epidermal sheets (Holzmann et al, 2004). It has also been used in immunoprecipitation analysis of cell lysates from lung cDCs and MC/9 mast cells (Grayson et al, 2007). In vitro incubation of basophils with this antibody blocks subsequent MAR-1 staining, but does not activate basophil IL-4 production (Sokol et al, 2008). Intravenous treatment of mice with this antibody results in basophil depletion in peripheral blood, spleen, bone marrow and liver, but not skin or intraperitoneal mast cell depletion (Sokol et al, 2008). Following treatment with this antibody, basophil-depleted mice display impaired Th2 differentiation upon papain immunisation (Sokol et al, 2008). However, MAR-1 treated mice respond normally to high-dose intravenous Ig in both the K/BxN serum transfer arthritis and collagen-induced arthritis models, despite basophil depletion (Campbell et al, 2014).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: Fc receptor, IgE, high affinity I, alpha polypeptide
• Database Link:
  Entrez Gene 14125 Mouse
  UniProt ID P20489
• Synonyms: FCE1A; FcERI