Protein C (PROC) Rabbit Monoclonal Antibody [Clone ID: HPC-4]

CAT#: TA386263

Recombinant Anti-Protein C (Clone HPC-4)

Specifications

Product Data

• Clone Name: HPC-4
• Applications: Bl, ELISA
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising mice with human protein C.
• Specificity: Specifically binds to a specific twelve peptide sequence (EDQVDPRLIDGK) in the activation region of the Protein C (spanning the thrombin cleavage site). Does not react with activated protein C. Has been shown to be cross-reactive with baboon Protein C but not with bovine or mouse Protein C.
  
  HPC-4 binds human protein C only in the presence of Ca2, it binds to the reduced carboxymethylated protein C heavy chain and it blocks protein C activation but does not bind or inactivate protein C or activated protein C bound to its plasma inhibitor (Taylor et al., 1987). It is an anti-epitope tag antibody, as it recognises EDQVDPRLIDGK sequence (Taylor et al., 1987). Furthermore, it has been shown to work in ELISAs to detemine expression levels (Velander et al., 1992). HPC-4 has been shown to be effective at purifying proteins tagged with its epitope (Rezaie et al., 1992). Has been used to block the activation of Protein C by the thrombin-thrombomodullin complex (Stearns et al., 1988). It was used for the same purpose in Baboons to lower the threshold of sepsis (Taylor Jr et al., 1987). HPC-4 has been used in many other studies to observe the function of Protein C by knocking out its function in a Ca2+ dependent manner, purificaion and others (Bae et al., 2007; Tanaka et al., 2005).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: protein C, inactivator of coagulation factors Va and VIIIa
• Database Link:
  Entrez Gene 5624 Human
  UniProt ID P04070
• Synonyms: APC; PC; PROC1