RHD Rabbit Monoclonal Antibody [Clone ID: D7C2]

CAT#: TA386267

Recombinant Anti-Rhesus D antigen (Clone D7C2)

Product Images

Flow cytometry using the Anti-Rhesus D antigen antibody D7C2 (Ab01214). Paraformaldehyde fixed K562 cells were stained with anti-unknown specificity antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of D7C2 (TA386267, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: D7C2
• Applications: FC
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Clonality: Monoclonal
• Immunogen: A Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r) and the peripheral lymphocytes were isolated and transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
• Specificity:
  
  FC confirmed the binding of D7C2 to Rh D+ red cells in artifical mixtures (Edelman et al, 1997). The ADCC of D7C2 showed a percentage of 93% lysis (Edelman et al, 1997). D7C2 has been expressed recombinantly using a baculovirus-insect cell expression system (Edelman et al, 1997).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: Rh blood group D antigen
• Database Link:
  Entrez Gene 6007 Human
  UniProt ID Q02161
• Synonyms: CD240D; DIIIc; MGC165007; RH; Rh4; RH30; Rh30a; RHCED; RhDCw; RHDel; RHDVA(TT); RhII; RhK562-II; RhPI; RHPII; RHXIII