IL9 Rabbit Monoclonal Antibody [Clone ID: MH9A4]

CAT#: TA386284

Recombinant Anti-IL-9 (Clone MH9A4)

Product Images

Flow-cytometry using the Anti-IL-9 antibody MH9A4 (Ab01261). Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MH9A4 (TA386284, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

Specifications

Product Data

• Clone Name: MH9A4
• Applications: ELISA, FC
• Reactivities: Human
• Modifications: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
• Host: Rabbit
• Isotype: IgG, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising C57Bl/6 mice with human IL-9 coupled to ovalbumin.
• Specificity: This antibody is specific for human IL-9.
  
  This antibody has been used in ELISA analysis to detect human IL-9 in allergen-stimulated peripheral blood mononuclear cell supernatants (Jenmalm et al, 2001) and differentiated human T helper cell supernatants (Chang et al, 2010). This antibody, when fluorochrome-conjugated, has also been used to achieve intracellular IL-9 staining, analysed using flow cytometry (Chang et al, 2010; Beriou et al, 2010; Lozano et al, 2013).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: interleukin 9
• Database Link:
  Entrez Gene 3578 Human
  UniProt ID P15248
• Synonyms: HP40; IL-9; P40