Lag3 Rat Monoclonal Antibody [Clone ID: C9B7W]

CAT#: TA386297

Recombinant Anti-Lag3 (Clone C9B7W)

Specifications

Product Data

• Clone Name: C9B7W
• Applications: Bl, FC, IP
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: This antibody was raised by immunising Lewis rats with a murine LAG-3:Ig fusion protein.
• Specificity: This antibody is specific for an epitope in the D2 domain of murine LAG-3.
  
  This antibody has been used in flow cytometric analysis of splenocytes (Workman et al, 2005), T cell hybridomas expressing wild‐type or chimeric CD4:murine LAG-3 (Workman et al, 2002) and CD4+ regulatory T cells (Chien et al, 2017). It has also been used in immunoprecipitation analysis of murine T hybridoma 3A9 cells transduced with murine LAG-3 (Li et al, 2004). This antibody blocks LAG-3 function in vitro, inhibiting antigen‐induced IL‐2 production in LAG-3-expressing T cell hybridomas, but does not block LAG-3:MHC class II interaction (Workman et al, 2002). Treatment with this antibody also blocks LAG-3 function in vivo, leading to accelerated homeostatic expansion of WT T cells (Workman et al, 2005). Administration of this LAG-3-blocking antibody, in combination with PD-L1 blockade, synergistically improves the survival of lymphodepleted myeloma-bearing mice (Jing et al, 2015).
• Formulation: PBS with 0.02% Proclin 300.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles.
• Stability: 3 years from dispatch.
• Gene Name: lymphocyte-activation gene 3
• Database Link:
  Entrez Gene 16768 Mouse
  UniProt ID Q61790
• Synonyms: CD223; FDC; LAG-3