MARCO Rabbit Monoclonal Antibody [Clone ID: PLK1]
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Specifications
Product Data | |
Clone Name | PLK1 |
Applications | FC, IF, IHC, IP, WB |
Reactivities | Human |
Modifications | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques. |
Host | Rabbit |
Isotype | IgG, kappa |
Clonality | Monoclonal |
Immunogen | The antibody was raised by immunising BALB/c mice with human alveolar macrophages (AMs) and subsequent creation of hybridomas. |
Specificity | This antibody recognises the extracellular domain V between residues 420 and 431 in MARCO both in human and bovine samples. The specificity of this antibody was confirmed in an IF experiment where the antibody stained alveolar macrophages for MACRO (Arredouani et al., 2005). Tissue samples prepared in acetone and paraformaldehyde were also successfully immunohistochemically stained (Arredouani et al., 2005; Martens et al., 2006). The antibody was also capable of weak staining of alveolar macrophages in flow cytometry (Palecanda and Kobzik, 2001). In IP experiments, the antibody immunoprecipitates MARCO from lysates obtained from COS cells transfected with human MARCO (Arredouani et al., 2005). |
Formulation | PBS with 0.02% Proclin 300. |
Concentration | lot specific |
Conjugation | Unconjugated |
Storage | Please store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. Avoid freeze and thaw cycles. |
Stability | 3 years from dispatch. |
Gene Name | macrophage receptor with collagenous structure |
Database Link | |
Synonyms | SCARA2 |
Reference Data |
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