Il12a Rat Monoclonal Antibody

CAT#: TA388904

IL-12 Antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: In a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Murine IL-12 when used with ProSci’s Biotinylated Antigen Affinity Purified anti-Murine IL-12 as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml.
  Western Blot
  To detect Murine IL-12 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IL-12 is 1.0-2.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.
• Reactivities: Mouse
• Host: Rat
• Clonality: Monoclonal
• Immunogen: Produced in Sprague Dawley rats immunized with highly pure Recombinant Murine IL-12. Anti-Murine IL-12-specific antibody was purified from cell culture by Protein G affinity chromatography.
• Concentration: lot specific
• Purification: IL-12-specific antibody was purified from cell culture by Protein G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interleukin 12a
• Database Link:
  Entrez Gene 16159 Mouse
  UniProt ID P43431
• Background: IL-12 is a potent regulator of cell-mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes and connective tissue-type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells, and induces the development of IFN-gamma-producing Th1 cells. Recombinant Murine IL-12 is a 75.0 kDa heterodimeric glycoprotein consisting of disulfide-linked 35 kDa (p35) and 40 kDa (p40) subunits (506 total amino acid residues).
• Synonyms: CLMF; IL-12; IL-12A; IL12; NFSK; NKSF1; P35