IL1 beta (IL1B) Goat Polyclonal Antibody

CAT#: TA388907

IL-1 beta Antibody (biotin)


USD 350.00

5 Days*

Size
    • 25 ug

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Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Sandwich ELISA: To detect hIL-1 beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human IL-1 beta as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hIL-1 beta.
Western Blot
To detect hIL-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Goat
Clonality Polyclonal
Immunogen Produced from sera of Goats pre-immunized with highly pure (>98%) recombinant hIL-1 beta. Anti-HumanIL-1beta specific antibody was purified by affinity chromatography and then biotinylated.
Concentration lot specific
Purification IL-1 beta specific antibody was purified by affinity chromatography and then biotinylated
Conjugation Biotin
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name interleukin 1 beta
Background IL-1 beta is a proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes, and other epithelial cells. Both IL-1 alpha and IL-1 beta bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. Recombinant Human IL-1 beta is a 17.3 kDa protein containing 153 amino acid residues.
Synonyms catabolin; IL-1; IL-1B; IL1-BETA; IL1F2; pro-interleukin-1-beta
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.