LTA Rabbit Polyclonal Antibody

CAT#: TA388911

TNF-beta Antibody

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect Human TNF-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human TNF-beta as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-beta.
  Western Blot
  To detect Human TNF-beta by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-beta is 1.5-3.0 ng/lane, under either reducing non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure recombinant Human TNF-beta. Anti-Human TNF-beta specific antibody was purified by affinity chromatography employing immobilized Human TNF-beta matrix.
• Concentration: lot specific
• Purification: TNF-beta specific antibody was purified by affinity chromatography employing immobilized Human TNF-beta matrix
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lymphotoxin alpha
• Database Link:
  Entrez Gene 4049 Human
  UniProt ID P01374
• Background: TNF-beta is a potent mediator of inflammatory and immune responses. It belongs to the TNF family of ligands, and signals through TNFR1 and TNFR2. TNF-beta is produced by activated T and B lymphocytes, and has similar activities to TNF-alpha. Like TNF-alpha, TNF-beta is involved in the regulation of various biological processes, including cell proliferation, differentiation, apoptosis, lipid metabolism, coagulation, and neurotransmission. TNF-beta is secreted as a soluble polypeptide, but can form heterotrimers with lymphotoxin-beta, which effectively anchors the TNF-beta to the cell surface. TNF-beta is cytotoxic to a wide range of tumor cells. Recombinant Human TNF-beta is a secreted 172 amino acid protein (18.6 kDa) which forms an active non-disulfide-linked homotrimer structure in solution.
• Synonyms: LT; LT-alpha; OTTHUMP00000165897; TNF-beta; TNFB; TNFSF1