Spasmolytic Polypeptide (TFF2) Rabbit Polyclonal Antibody

CAT#: TA388946

TFF-2 Antibody (biotin)

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect Human TFF-2 by sandwich ELISA (using 100 ul/well) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti- Human TFF-2 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human TFF-2.
  Western Blot
  To detect Human TFF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human TFF-2 is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits immunized with highly pure recombinant Human TFF-2. Anti-Human TFF-2 specific antibody was purified by affinity chromatography and then biotinylated.
• Concentration: lot specific
• Purification: TFF-2 specific antibody was purified by affinity chromatography and then biotinylated
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: trefoil factor 2
• Database Link:
  Entrez Gene 7032 Human
  UniProt ID Q03403
• Background: The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF2 has been shown to inhibit gastrointestinal motility and gastric acid secretion. Recent data suggests a potential role for TFF2 in acute and chronic asthma (Nikolaidis, N.M. et al. Am. Journal Respir. Cell Mol. Biol. (2003) 4: 458-464). Recombinant Human TFF-2 is a 12.0 kDa polypeptide of 107 amino acid residues, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
• Synonyms: SML1; SP; spasmolysin