Phospho-PTK2 (pTyr397) Mouse Antibody [Clone ID: M121]

CAT#: TA389133

Anti-FAK (Tyr-397), Phosphospecific Antibody

Specifications

Product Data

• Clone Name: M121
• Applications: WB
• Recommended Dilution: WB: 1:500
• Reactivities: Human, Mouse, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Immunogen: Clone (M121) was generated from a synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 397 of human FAK. This peptide sequence has high homology to the conserved tyrosine site in rat and mouse FAK.
• Specificity: The antibody detects a 125 kDa* protein on SDS-PAGE immunoblots of untreated HUVEC cells. This phosphorylated band is greatly reduced after treatment with alkaline phosphatase.
• Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
• Concentration: lot specific
• Purification: Protein A Purified
• Conjugation: Unconjugated
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Stability: After date of receipt, stable for at least 1 year at -20°C.
• Predicted Protein Size: 125
• Database Link:
  UniProt ID Q05397
• Background: Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in signal transduction pathways important for cell spreading, migration and survival. Activation of FAK by integrin clustering leads to autophosphorylation at Tyr-397, which is a binding site for Src family kinases, PI3-Kinase, and PLCγ. The recruitment of Src family kinases results in the phosphorylation of tyrosine 407, 576, and 577 in the catalytic domain, and tyrosine 871 and 925 in the carboxy-terminal region of FAK. Thus, the phosphorylation of Tyr-397 is a critical step in the activation of FAK.
• Note: Protein G purified tissue culture supernatant.