BTK Rabbit Polyclonal Antibody

CAT#: TA392899

BTK (phospho-Y551) polyclonal antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:500~1:1000
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human BTK around the phosphorylation site of Tyrosine 551.
• Specificity: BTK (phospho-Y551) polyclonal antibody detects endogenous levels of BTK protein only when phosphorylated at Tyr551.
• Formulation: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Concentration: 1mg/ml
• Conjugation: Unconjugated
• Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Stability: 1 year
• Predicted Protein Size: ~ 77 kDa
• Gene Name: Bruton tyrosine kinase
• Database Link:
  Entrez Gene 695 Human
  UniProt ID Q06187
• Background: Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. Like other BTK family members, it contains a pleckstrin homology (PH) domain, Src homology SH3 and SH2 domains. BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of BTK is negatively regulated by PKC beta through phosphorylation of BTK at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation.
• Synonyms: Agammaglobulinemia tyrosine kinase; AGMX1; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase; BTK; Tyrosine-protein kinase BTK
• Note: For research use only, not for use in diagnostic procedure.