Edc3 Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, WB |
Recommended Dilution | WB: 0.5ug/ml ELISA: 1: 10,000 |
Reactivities | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | EDC3 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic phospho-peptide surrounding the pS161 site of mouse EDC3. |
Specificity | Anti-EDC3 pS161 is specific for phospho S161 mouse EDC3. It was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with rabbit, pig, monkey, bovine, rat, mouse, and bat based on 100% sequence homology. Cross-reactivity with EDC3 pS161 from other sources has not been determined. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Concentration | 1.0 mg/ml |
Conjugation | Unconjugated |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Stability | Expiration date is one (1) year from date of receipt. |
Database Link | |
Background | EDC3 antibody is associated with an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end. It has also been implicated in cell signaling involving the 14-3-3 proteins and related pathways. Anti-EDC3 [pS161] antibody is ideal for investigators interested in Epigenetic and Nuclear Signaling research. |
Synonyms | rabbit anti-EDC3 pS161 Antibody, Enhancer of mRNA-decapping protein 3, YjeF domain-containing protein 1, Edc-3, Edc 3, Yjdc |
Note | Anti-EDC3pS161 antibody is useful for ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~55kDa corresponding to the appropriate cell lysate or extract. |
Reference Data |
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