ICAD (DFFA) Mouse Monoclonal Antibody [Clone ID: OTI1B10]

CAT#: TA500061

Anti-DFF45 (ICAD) mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DFFA (RC202879, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DFFA. Positive lysates LY418008 (100ug) and LC418008 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DFFA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  DFF45 antibody (1B10) at 1:500 dilution + Hela cell lysate

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  Anti-DFFA mouse monoclonal antibody (TA500061) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY DFFA (RC202879)

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  Immunofluorescent staining of HeLa cells using anti-DFFA mouse monoclonal antibody (TA500061).

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Specifications

Product Data

• Clone Name: OTI1B10
• Applications: IF, WB
• Recommended Dilution: WB: 1:500, IF (1:100)
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 1-331 of human DFF45
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.3 kDa
• Gene Name: DNA fragmentation factor subunit alpha
• Database Link:
  NP_004392
  Entrez Gene 1676 Human
  UniProt ID O00273
• Background: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene
• Synonyms: DFF-45; DFF1; ICAD

Reference Data

• Protein Pathways: Apoptosis