Melanoma gp100 (PMEL) Mouse Monoclonal Antibody [Clone ID: OTI9G3]

CAT#: TA500400

Anti-SILV (Melanoma gp100) mouse monoclonal antibody, clone OTI9G3 (formerly 9G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SILV (RC200663, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SILV. Positive lysates LY402062 (100ug) and LC402062 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SILV monoclonal antibody.

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SILV mouse monoclonal antibody. (TA500400)

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SILV mouse monoclonal antibody. (TA500400)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SILV mouse monoclonal antibody. (TA500400)

• 

  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SILV mouse monoclonal antibody. (TA500400)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SILV mouse monoclonal antibody. (TA500400)

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SILV mouse monoclonal antibody. (TA500400)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SILV mouse monoclonal antibody. (TA500400)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SILV mouse monoclonal antibody. (TA500400)

• Anti-SILV mouse monoclonal antibody (TA500400) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SILV (RC200663).

Show all 11 images

Specifications

Product Data

• Clone Name: OTI9G3
• Applications: IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC: 1:150
• Reactivities: Human, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human SILV expression vector
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 1.9 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.3 kDa
• Gene Name: premelanosome protein
• Database Link:
  NP_008859
  Entrez Gene 481102 DogEntrez Gene 6490 Human
  UniProt ID P40967
• Background: Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity
• Synonyms: D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV

Reference Data

• Protein Families: Secreted Protein, Transmembrane