Melanoma gp100 (PMEL) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI7E3]

CAT#: TA500425AM

SILV (Melanoma gp100) mouse monoclonal antibody, clone OTI7E3 (formerly 7E3), Biotinylated

Product Images

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SILV mouse monoclonal antibody. (TA500425)

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  HEK293T cells transfected with either RC200663 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SILV antibody (TA500425), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI7E3
• Applications: FC, IHC
• Recommended Dilution: FLOW 1:100, IHC: 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SILV (NP_008859) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70.3 kDa
• Gene Name: premelanosome protein
• Database Link:
  NP_008859
  Entrez Gene 6490 Human
  UniProt ID P40967
• Background: Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
• Synonyms: D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL17; SI; SIL; SILV

Reference Data

• Protein Families: Secreted Protein, Transmembrane