Glucose Transporter 5 GLUT5 (SLC2A5) Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI9F3]

CAT#: TA500556AM

SLC2A5 (GLUT5) mouse monoclonal antibody, clone OTI9F3 (formerly 9F3), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5. Positive lysates LY401061 (100ug) and LC401061 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-SLC2A5 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-SLC2A5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500556)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SLC2A5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500556)

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-SLC2A5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500556)

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  Immunoprecipitation (IP) of SLC2A5 by using TrueMab monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of DDK tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC2A5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• Flow cytometric Analysis of Hela cells, using anti-SLC2A5 antibody (TA500556), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-SLC2A5 antibody (TA500556), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI9F3
• Applications: FC, IHC, IP, WB
• Recommended Dilution: WB 1:500~1000, IHC 1:50, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.8 kDa
• Gene Name: solute carrier family 2 member 5
• Database Link:
  NP_003030
  Entrez Gene 722154 MonkeyEntrez Gene 6518 Human
  UniProt ID P22732
• Synonyms: GLUT-5; GLUT5

Reference Data

• Protein Families: Transmembrane