Glucose Transporter 5 GLUT5 (SLC2A5) Mouse Monoclonal Antibody [Clone ID: OTI12F2]

CAT#: TA500575S

SLC2A5 (GLUT5) mouse monoclonal antibody, clone OTI12F2 (formerly 12F2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC2A5 (RC200418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SLC2A5. Positive lysates LY401061 (100ug) and LC401061 (20ug) can be purchased separately from OriGene.

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  Immunofluorescent staining of HT29 cells using anti-SLC2A5 mouse monoclonal antibody (TA500575).

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  Immunoprecipitation (IP) of SLC2A5 by using TrueMab monoclonal anti-SLC2A5 antibodies (Negative control: IP without adding anti-SLC2A5 antibody.). For each experiment, 500ul of DDK tagged SLC2A5 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SLC2A5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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  HEK293T cells transfected with either RC200418 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SLC2A5 antibody (TA500575), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI12F2
• Applications: FC, IF, IP, WB
• Recommended Dilution: WB 1:1000, IF 1:50, FLOW 1:100, IP 2ug/500ul
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SLC2A5 (NP_003030) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.6 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.8 kDa
• Gene Name: solute carrier family 2 member 5
• Database Link:
  NP_003030
  Entrez Gene 6518 Human
  UniProt ID P22732
• Synonyms: GLUT-5; GLUT5

Reference Data

• Protein Families: Transmembrane