BubR1 (BUB1B) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI6E3]

CAT#: TA500679BM

BuB1B (BubR1) mouse monoclonal antibody, clone OTI6E3 (formerly 6E3), HRP conjugated

Product Images

Anti-BUB1B mouse monoclonal antibody (TA500679) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B (RC205844).

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BUB1B monoclonal antibody.

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B. Positive lysates LY420068 (100ug) and LC420068 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of Jurkat cells, using anti-BUB1B antibody (TA500679), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-BUB1B antibody (TA500679), (Red), compared to a nonspecific negative control antibody ([TA50011]), (Blue).

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  HEK293T cells transfected with either RC205844 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BUB1B antibody (TA500679), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI6E3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BUB1B (NP_001202) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 119.3 kDa
• Gene Name: BUB1 mitotic checkpoint serine/threonine kinase B
• Database Link:
  NP_001202
  Entrez Gene 171576 RatEntrez Gene 608350 DogEntrez Gene 100423079 MonkeyEntrez Gene 701 Human
  UniProt ID O60566
• Background: This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq]
• Synonyms: Bub1A; BUB1beta; BUBR1; hBUBR1; MAD3L; MVA1; SSK1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle