ERCC1 Mouse Monoclonal Antibody [Clone ID: OTI1C6]

CAT#: TA501169S

Anti-ERCC1 mouse monoclonal antibody, clone OTI1C6 (formerly 1C6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERCC1 (RC200478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERCC1. Positive lysates LY419605 (100ug) and LC419605 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501169, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501169, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501169, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501169, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ERCC1 mouse monoclonal antibody at 1:150 (TA501169)

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody at 1:150 (TA501169)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ERCC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501169, Dilution 1:50)

• Anti-ERCC1 mouse monoclonal antibody (TA501169) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERCC1 (RC200478).

• HEK293T cells transfected with either RC200478 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ERCC1 antibody (TA501169), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-ERCC1 antibody (TA501169), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-ERCC1 antibody (TA501169), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1C6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IF: 1:100, FLOW: 1:100, IHC: 1:50-1:150
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ERCC1 (NP_973730) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.51 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.4 kDa
• Gene Name: ERCC excision repair 1, endonuclease non-catalytic subunit
• Database Link:
  NP_001974
  Entrez Gene 13870 MouseEntrez Gene 2067 Human
  UniProt ID P07992
• Background: The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand. [provided by RefSeq]
• Synonyms: COFS4; RAD10; UV20

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair