RBBP9 Mouse Monoclonal Antibody [Clone ID: OTI4G9]

CAT#: TA501741S

Anti-RBBP9 mouse monoclonal antibody, clone OTI4G9 (formerly 4G9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBBP9 (RC202090, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBBP9. Positive lysates LY401976 (100ug) and LC401976 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RBBP9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501741, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RBBP9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501741, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-RBBP9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501741, Dilution 1:50)

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  Anti-RBBP9 mouse monoclonal antibody (TA501741) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RBBP9 (RC202090).

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  HEK293T cells transfected with either RC202090 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RBBP9 antibody (TA501741), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4G9
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, Flow: 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RBBP9 (NP_006597) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.67 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 20.8 kDa
• Gene Name: RB binding protein 9, serine hydrolase
• Database Link:
  NP_006597
  Entrez Gene 10741 Human
  UniProt ID O75884
• Background: The protein encoded by this gene is a retinoblastoma binding protein that may play a role in the regulation of cell proliferation and differentiation. Two alternatively spliced transcript variants of this gene with identical predicted protein products have been reported, one of which is a nonsense-mediated decay candidate. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: BOG; RBBP10