MMAB Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI5G1]

CAT#: TA502264AM

MMAB mouse monoclonal antibody, clone OTI5G1 (formerly 5G1), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMAB (RC204290, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMAB. Positive lysates LY409455 (100ug) and LC409455 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MMAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502264)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MMAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502264)

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  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MMAB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502264)

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  HEK293T cells transfected with either RC204290 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MMAB antibody (TA502264), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5G1
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MMAB (NP_443077) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.0 kDa
• Gene Name: metabolism of cobalamin associated B
• Database Link:
  NP_443077
  Entrez Gene 326625 Human
  UniProt ID Q96EY8
• Background: This gene encodes a protein that catalyzes the final step in the conversion of vitamin B(12) into adenosylcobalamin (AdoCbl), a vitamin B12-containing coenzyme for methylmalonyl-CoA mutase. Mutations in the gene are the cause of vitamin B12-dependent methylmalonic aciduria linked to the cblB complementation group. [provided by RefSeq]
• Synonyms: ATR; cblB; CFAP23; cob

Reference Data

• Protein Pathways: Metabolic pathways, Porphyrin and chlorophyll metabolism