POLR2E Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI3C5]

CAT#: TA502488BM

POLR2E mouse monoclonal antibody, clone OTI3C5 (formerly 3C5), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2E (RC201266, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2E. Positive lysates LY419161 (100ug) and LC419161 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-POLR2E monoclonal antibody.

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  Anti-POLR2E mouse monoclonal antibody (TA502488) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2E (RC201266).

Specifications

Product Data

• Clone Name: OTI3C5
• Applications: IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human POLR2E (NP_002686) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.4 kDa
• Gene Name: RNA polymerase II, I and III subunit E
• Database Link:
  NP_002686
  Entrez Gene 66420 MouseEntrez Gene 690966 RatEntrez Gene 5434 Human
  UniProt ID P19388
• Background: This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]
• Synonyms: hRPB25; hsRPB5; RPABC1; RPB5; XAP4

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase