HDHD1A (PUDP) Mouse Monoclonal Antibody [Clone ID: OTI2E10]

CAT#: TA502621

HDHD1A mouse monoclonal antibody, clone OTI2E10 (formerly 2E10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDHD1A (RC229126, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDHD1A. Positive lysates LY415984 (100ug) and LC415984 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HDHD1A monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-HDHD1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502621)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HDHD1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502621)

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  HEK293T cells transfected with either RC204419 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDHD1A antibody (TA502621), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2E10
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Monkey, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HDHD1A (NP_036212) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.53 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.5 kDa
• Gene Name: pseudouridine 5'-phosphatase
• Database Link:
  NP_036212
  Entrez Gene 291585 RatEntrez Gene 610678 DogEntrez Gene 707930 MonkeyEntrez Gene 8226 Human
  UniProt ID Q08623
• Background: This gene encodes a member of the haloacid dehalogenase-like (HAD) hydrolase superfamily. The encoded protein has no known biological function. This gene has a pseudogene on chromosome 1. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]
• Synonyms: DXF68S1E; FAM16AX; GS1; HDHD1A