NDUFA7 Mouse Monoclonal Antibody [Clone ID: OTI2G4]

CAT#: TA502658

NDUFA7 mouse monoclonal antibody, clone OTI2G4 (formerly 2G4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFA7 (RC200534, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFA7. Positive lysates LY417603 (100ug) and LC417603 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NDUFA7 monoclonal antibody.

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  Anti-NDUFA7 mouse monoclonal antibody (TA502658) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NDUFA7 (RC200534).

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  HEK293T cells transfected with either RC200534 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NDUFA7 antibody (TA502658), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-NDUFA7 antibody (TA502658), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-NDUFA7 antibody (TA502658), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI2G4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human NDUFA7 (NP_004992) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.62 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.4 kDa
• Gene Name: NADH:ubiquinone oxidoreductase subunit A7
• Database Link:
  NP_004992
  Entrez Gene 66416 MouseEntrez Gene 299643 RatEntrez Gene 4701 Human
  UniProt ID O95182
• Synonyms: B14.5a; CI-B14.5a

Reference Data

• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease