PPM1B Mouse Monoclonal Antibody (Biotin conjugated) [Clone ID: OTI3F2]

CAT#: TA502865AM

PPM1B mouse monoclonal antibody, clone OTI3F2 (formerly 3F2), Biotinylated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B (RC212918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1B. Positive lysates LY419156 (100ug) and LC419156 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPM1B monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PPM1B-Knockout 293T cells (KO, Cat# LC811002) were separated by SDS-PAGE and immunoblotted with anti-PPM1B monoclonal antibody TA502865, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  HEK293T cells transfected with either RC212918 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPM1B antibody (TA502865), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-PPM1B antibody (TA502865), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-PPM1B antibody (TA502865), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3F2
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPM1B (NP_002697) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Biotin
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.5 kDa
• Gene Name: protein phosphatase, Mg2+/Mn2+ dependent 1B
• Database Link:
  NP_002697
  Entrez Gene 19043 MouseEntrez Gene 24667 RatEntrez Gene 474573 DogEntrez Gene 713918 MonkeyEntrez Gene 5495 Human
  UniProt ID O75688
• Synonyms: PP2C-beta; PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Stem cell - Pluripotency
• Protein Pathways: MAPK signaling pathway