LIM Kinase 1 (LIMK1) Mouse Monoclonal Antibody (HRP conjugated) [Clone ID: OTI8C8]

CAT#: TA502978BM

LIMK1 (LIM Kinase 1 ) mouse monoclonal antibody, clone OTI8C8 (formerly 8C8), HRP conjugated

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIMK1 (RC218058, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIMK1. Positive lysates LY400838 (100ug) and LC400838 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502978)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-LIMK1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502978)

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  Anti-LIMK1 mouse monoclonal antibody (TA502978) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIMK1 (RC218058).

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  HEK293T cells transfected with either RC218058 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIMK1 antibody (TA502978), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-LIMK1 antibody (TA502978) (Red), compared to a nonspecific negative control antibody (Blue).

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Specifications

Product Data

• Clone Name: OTI8C8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LIMK1(NP_002305) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.5 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: HRP
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.4 kDa
• Gene Name: LIM domain kinase 1
• Database Link:
  NP_002305
  Entrez Gene 16885 MouseEntrez Gene 65172 RatEntrez Gene 3984 Human
  UniProt ID P53667
• Background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Synonyms: LIMK; LIMK-1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton