Spermine synthase (SMS) Mouse Monoclonal Antibody [Clone ID: OTI5F2]

CAT#: TA503098

SMS (Spermine synthase) mouse monoclonal antibody, clone OTI5F2 (formerly 5F2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMS (RC200619, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMS. Positive lysates LY417877 (100ug) and LC417877 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SMS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503098)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SMS mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503098)

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  Anti-SMS mouse monoclonal antibody (TA503098) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SMS (RC200619).

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  Immunofluorescent staining of HeLa cells using anti-SMS mouse monoclonal antibody (TA503098).

• HEK293T cells transfected with either RC200619 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SMS antibody (TA503098), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5F2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:50~100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SMS (NP_004586) produced in HEK293T cell.
• Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.59 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.1 kDa
• Gene Name: spermine synthase
• Database Link:
  NP_004586
  Entrez Gene 20603 MouseEntrez Gene 363469 RatEntrez Gene 6611 Human
  UniProt ID P52788
• Background: This gene encodes a protein belonging to the spermidine/spermin synthase family. Pseudogenes of this gene are located on chromosomes 1, 5, 6 and X. Mutations in this gene are associated with X-linked Snyder-Robinson mental retardation syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
• Synonyms: MRSR; SPMSY; SpS; SRS

Reference Data

• Protein Pathways: Arginine and proline metabolism, beta-Alanine metabolism, Cysteine and methionine metabolism, Glutathione metabolism, Metabolic pathways